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. 2018 Mar 30;3(3):3608–3616. doi: 10.1021/acsomega.8b00220

Figure 3.

Figure 3

PIP-RBPJ-1 alters the expression of HES1 and its regulated downstream genes. (A) Effect of PIP-RBPJ-1 and PIP-RBPJ-2 on the HES1 expression. (B) Expression profile of HES1 in hNSCs treated with PIP-RBPJ-1 at different concentrations (0.5, 2, and 5 μM) for 24 h. (C) Effect of PIP-RBPJ-1 on the expression profile of HES1 at various time intervals (6, 12, 24, and 48 h). Expression profile of (D) ASCL1 and (E) NGN2 in PIP-RBPJ-1-treated hNSCs. The housekeeping gene β-actin is chosen to normalize the relative gene expression, as the expression of β-actin did not alter with both PIP-RBPJ-1 and PIP-RBPJ-2 treatment. Three biological replicates were performed, and the mean ± SD are indicated, *P < 0.05, **P < 0.01.