Table 1. Shift of Tm Value by RBPJ-Binding PIPsa.
PIP-RBPJ-1 |
PIP-RBPJ-2 |
PIP-C |
|||||
---|---|---|---|---|---|---|---|
Tm/°C | Tm/°C | ΔTm/°C | Tm/°C | ΔTm/°C | Tm/°C | ΔTm/°C | |
ODN-1 | 54.3(±0.1) | 80.4(±0.3) | 26.1 | 59.4(±0.2) | 5.1 | 56.5(±0.2) | 2.2 |
ODN-2 | 37.9(±0.1) | 56.7(±0.6) | 18.8 | 47.4(±0.1) | 9.6 | 47.0(±0.6) | 2.2 |
Melting temperatures were calculated and analyzed, and each standard deviation is indicated in parentheses. Tm = Tm (DNA complex compound) – Tm (DNA).