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Pathological lesions in H9C2 cells following heat stress for 0, 1, 3 and 5 h (bar = 20 μm) shown by haematoxylin and eosin staining. Heat exposure used granular degeneration (rightwards arrow) after 1 h, vacuolar degeneration (upwards arrow) after 3 h and karyopyknosis (downwards arrow) after 5 h in the control group. Supplementation with vitamin C or vitamin C-Na resulted in less granular degeneration and vacuolar degeneration after 1 and 3 h, and no karyopyknosis after 5 h
