Fig. 1.

UBI4-deleted cells are sensitive to PQ. Cell proliferation assays with wild-type and ubi4Δ cells in YPD medium with or without PQ were performed by the Bioscreen C MBR machine (a). *p < 0.05 indicated a significant difference. Wild-type and ubi4Δ cells were 5-fold serially diluted and spotted onto YPD plates or PQ-supplemented YPD plates. These plates were kept at 30 °C until colonies formed (b). pAUR123, empty plasmid; pAUR123UBI4, UBI4 overexpression plasmid