Fig. 7.

Overexpression of UBI4 increases yeast resistance to PQ. Cell proliferation assays for wild-type and UBI4OX cells in YPD medium with or without 2 mM PQ were performed by a Bioscreen C MBR machine (a). *p < 0.05 indicated a significant difference. Wild-type and UBI4OX cells were 5-fold serially diluted, spotted onto YPD or PQ-supplemented YPD plates. These plates were kept at 30 °C until colonies formed (b). RLS of UBI4OX cells (c). Mean lifespans and cell counts are shown in parentheses, and N is the total number of mother cells that were used for the RLS assay. Statistical significance was calculated by using the Wilcoxon rank-sum test, and *p < 0.05 indicated a significant difference