Fig. 8.

UBI4 overexpression increases ubiquitination under PQ-treated conditions. Changes in the levels of Ub monomers were evaluated by Western blotting (Tricine–SDS-PAGE) in lysates from yeast cells that had not been treated with PQ and had been treated with 2 mM PQ for 12 h (a). Changes in the levels of ubiquitinated proteins were evaluated by Western blotting (15% SDS-PAGE) using lysates from yeast cells that had not been treated with PQ and had been treated with 2 mM PQ for 12 h (b). Quantification of ubiquitin monomers and ubiquitinated proteins by densitometry