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. 2018 Jul 13;8:10672. doi: 10.1038/s41598-018-28770-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time-lapse intravital live imaging reveals the kinetics of VEGFA-induced permeability. (A-A’) Snapshots from live-imaged, tracer-injected corneal vessel networks, 5 or 50 minutes after topical application of PBS. The blue and magenta dashed boxes in A indicate low permeability vessels (blue) and high permeability vessels (magenta), respectively. (B) Quantification of relative change in extravasated tracer intensity over time comparing low permeability vessels and high permeability vessels in the PBS treated group (N.S. calculated by Two-way ANOVA). (C-C’) Snapshots from live-imaged, tracer-injected corneal vessel networks, 5 or 50 minutes after topical application of VEGFA. (D) Quantification of the relative change in interstitial (outside of lumen) tracer intensity over time, following application of VEGFA or PBS. p < 0.001, calculated by Two-way ANOVA. (E) Time course of the area indicated in C. Arrowheads point to sites of tracer extravasation.