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. 2018 Jul 13;8:10656. doi: 10.1038/s41598-018-28874-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neonatal sevo has a long-lasting effect on P7-P7 sevo-regulated miRNAs. (A) The P7-adult group represents mice that were treated at P7, followed by tissue collection when the mice were adults (B) In the whole brain, P7 sevo treatment has long-lasting effect on miR-145, miR29a, and miR300 (Two-way ANOVA, treatment, comparing no sevo from the combined P7-P7 and P7-adult vs. sevo from the combined P7-P7 and P7-adult, P < 0.05; posthoc, no sevo vs. sevo in the P7-adult, P < 0.05). (C) In the hippocampus, P7 sevo treatment has long-lasting effect on miR-20a, 20b, 532 and 99a. This effect was observed through the expression fold change, P7-adult/P7-P7 (t-test, no sevo vs. sevo, P < 0.05). * denotes P < 0.05.