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. 2018 Jul 13;8:10652. doi: 10.1038/s41598-018-28843-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Structure of predicted Rho-independent transcriptional terminator located in the untranslated penA upstream region. The predicted 49 nucleotide terminator sequence (TERM 264) is situated between nucleotides 25 and 73 upstream of the penA gene start codon in both 1026b (a) and Bp1651 (b). The G to A transition that generates the indicated −10 promoter sequence (underlined) at position −78 with respect to the start of penA is indicated in red type and marked by the arrow. The secondary structure numbering refers to the location of the respective bases in the 49 nucleotide terminator sequence. Gibbs free energy values for the secondary structures are shown in parentheses.