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. 2018 Jul 13;8:10652. doi: 10.1038/s41598-018-28843-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transposon T24 insertions in Bp1651 with decreased ceftazidime and meropenem resistance. Transposon insertions were mapped to the Bp1651 genome (GenBank: CP012041.1 and CP012042.1) by determining the transposon-chromosomal DNA junction sequences, followed by BLAST. The approximate T24 insertion sites in either chromosome 1 (C1) or 2 (C2) are indicated by the vertical arrowheads. Genes indicated in black were identified in mutants that did not grow on LB plates in the presence 16 μg/ml ceftazidime or 2 μg/ml meropenem. Genes indicated in white were identified in mutants that did not grow on LB plates containing meropenem, but grew on ceftazidime-containing plates.