Table 2.
Bp82 and derivatives used in this study.
| Strain | Relevant properties | Source |
|---|---|---|
| Bp82 | Attenuated ΔpurM derivative of 1026b | 43 |
| Bp82.87 | Δ(amrAB-oprA) | 8 |
| Strains used to assess promoter activity in penA ’ -lacZ fusions a,b | ||
| Bp82.363 | Bp82.87::mini-Tn7T-Gm-lacZ | This study |
| Bp82.364 | Bp82.87::mini-Tn7T-Gm-1026b-42 bp nlpD1-IR-penA’-lacZc | This study |
| Bp82.365 | Bp82.87::mini-Tn7T-Gm-Bp1651-42 bp nlpD1-IR-penA’-lacZ | This study |
| Bp82.366 | Bp82.87::mini-Tn7T-Gm-1026b-42 bp nlpD1-41T-IR-penA’-lacZ | This study |
| Bp82.367 | Bp82.87::mini-Tn7T-Gm-1026b-42 bp nlpD1-78A-IR-penA’-lacZ | This study |
| Bp82.371 | Bp82.87::mini-Tn7T-Gm-1026b-42 bp nlpD1-171C-IR-penA’-lacZ | This study |
| Bp82.372 | Bp82.87::mini-Tn7T-Gm-1026b-336 bp nlpD1-IR-penA’-lacZ | This study |
| Strains used to assess TERM 264 terminator activity b,d | ||
| Bp82.374 | Bp82.87::mini-Tn7T-Gm-134 bp-TERM 264-penA’-‘lacZ; | This study |
| Bp82.375 | Bp82.87::mini-Tn7T-Gm-134 bp-ΔTERM 264-penA’-‘lacZ | This study |
| Bp82.380 | Bp82.87::mini-Tn7T-Gm-24 bp-TERM 264-penA’-‘lacZ | This study |
| Bp82.381 | Bp82.87::mini-Tn7T-Gm-24 bp-ΔTERM 264-penA’-‘lacZ | This study |
aThe strains contained the mini-Tn7-penA’-lacZ fusion construct at attTn7 glmS3, except Bp82.371 and Bp82.372, which had the fusion constructs inserted at attTn7 glmS2.
bGm, gentamicin resistance marker.
cIR, nlpD1-penA intergenic region.
dAll strains contained the mini-Tn7-penA’-lacZ fusion construct at attTn7 glmS3.