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. 2018 Jul 13;8:10633. doi: 10.1038/s41598-018-28919-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GTM analysis applied to EPS production by B. breve. GTM applied to EPS production phenotype in B. breve. Panel A: Heatmap representing the EPS-associated genetic functions of which presence matched the observed EPS production phenotype (red) across the assessed B. breve strains. Five strains showing discrepant genotype/phenotype association are italicized. Panel B: EPS production phenotypic assay. Non-EPS-producers clearly show apparent reduction in OD as they ‘sediment’ to the bottom of the tube, while EPS producers do not exhibit such a sedimentation phenotype.