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. 2018 Jul 13;8:10668. doi: 10.1038/s41598-018-29048-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibitory effects of anisomycin on HCC cells. (a) Anisomycin chemical structure; percent cell proliferation inhibition caused by anisomycin treatment in (b) HepG2, (c) Huh7, and (d) SNU449 cells was determined, and data were pooled from three independent experiments. The indicated IC₅₀ values were estimated from the concentration-response curve. (e) HepG2, Huh7, and SNU449 cells were treated with DMSO (control) or 0.2 µM anisomycin for 48 h, and photographs were taken under a light microscope. Representative images from more than three independent experiments are shown.