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. 2018 Jul 13;8:10668. doi: 10.1038/s41598-018-29048-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NK cell-dependent effects of anisomycin in an HCC xenograft mouse model. (a) Schematic plot of the study design and route of injection for therapeutic efficacy. (b) Five days after inoculation of HepG2 cells, anisomycin (10 mg/kg) was administered from days 0 to 5 and from days 15 to 20 after initiation of treatment via the intraperitoneal (i.p) route. NK cells (5 × 10⁶ cells/mouse) were transferred into mice two times on days 6 and 11 during the treatment pause period, as described in the Materials and Methods (n = 6 mice). Tumour sizes were measured on the indicated days. (c) Tumours in each group of mice (n = 6) on day 23 of the experiment. (d) Body weights of each group of mice (n = 6) were measured every 3 days.