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. 2018 Jul 13;9:2710. doi: 10.1038/s41467-018-04654-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — IPN inputs to LDTg are concentration-dependently modulated by nicotine via β2-containing nAChRs. a Left: example time course, normalized oIPSC amplitudes (30 ms, 50 pA scale). Right: representative traces (black: baseline, color: nicotine). b Normalized average oIPSC amplitudes, baseline (gray) vs. nicotine (colored); ratio paired t-tests (one-tailed), 10 μM: t₈ = 3.492, P = 0.0041, n = 9 cells from 6 mice; 1 μM: t₆ = 3.367, P = 0.0075, n = 7 cells from 3 mice; 100 nM: t₆ = 1.708, P = 0.0692, n = 7 cells from 3 mice. c Prevalence of increase in oIPSC amplitudes; chi-square (two-sided), Z_{6.112, 1} = 2.472, P = 0.0134, n = 9, n = 7 cells. d Representative PPR traces (50 ms, 25 pA scale). e Summary PPR graph; unpaired t-test (one-sided), t₁₂ = 1.804, P = 0.0482, n = 7 cells from 3 mice. f Upper: Representative mIPSC traces (50 ms, 50 pA scale). Lower: Representative histograms of mIPSC frequency. g Normalized average mIPSC frequencies, baseline (gray) vs. nicotine (colored); ratio paired t-tests (one-tailed), 10 μM: t₈ = 3.33, P = 0.0052, n = 9 from 7 mice; 1 μM: t₇ = 3.176, P = 0.0078, n = 8 cells from 3 mice; 100 nM: t₇ = 1.546, P = 0.0830, n = 8 cells from 4 mice. h Prevalence of increase in mIPSC frequency; chi-square test (one-sided), Z_{2.951, 1} = 1.718, P = 0.0429, n = 9, n = 8 cells. i Upper: Representative cumulative amplitude histogram, baseline (black) vs. 10 μM nicotine (red). Lower: Normalized average mIPSC amplitudes, baseline (gray) vs. nicotine (colored). j Upper: 10 μM nicotine vs. 10 μM nicotine application + BAPTA in recording pipette; unpaired t-test (one-tailed), t₁₇ = 1.012, P = 0.1629, n = 9 cells from 6 mice, n = 10 cells from 5 mice. Lower: Prevalence of mIPSC frequency increase; chi-square test (one-sided), Z_{0.09048, 1} = 0.3008, P = 0.3818, n = 9, n = 10 cells. k Normalized average mIPSC frequencies, 10 μM nicotine vs. 10 μM nicotine + DhβE or MEC; one-way ANOVA, y = log(y) transform, F_{2, 26} = 6.175, P = 0.0064 (main effect), Holm–Sidak P = 0.3830, P = 0.0048, for DhβE or MEC, respectively. l Prevalence of mIPSC frequency increase; chi-square test (one-sided), Z_{3.104, 1} = 1.762, P = 0.0391, n = 9 cells from 2 mice, n = 11 cells from 3 mice. m Normalized average mIPSC frequencies, 10 μM nicotine vs. 10 μM + SR or 10 μM + αBTX; n = 9, n = 7 cells from 2 mice, n = 6 cells from 2 mice. n Prevalence of mIPSC frequency increase. Data presented as mean ± SEM, * P< 0.05