Fig. 4.

Inhibition of IPN terminals in LDTg blocks nicotine conditioned place aversion. a Upper: experimental timeline. Lower left: schematic of viral expression and fiber implants. Right: functional expression of ARCH, (500 ms, 100 pA; 2 sec, 5 mV scale). b Representative plots of animal position during post-test. c Normalized average preference scores; two-way RM ANOVA with Bonferroni post-hoc: P = 0.0022, EYFP n = 13 mice; ARCH n = 8 mice. d Change in preference for nicotine-paired side after conditioning: unpaired t-test (two-tailed), t₁₉ = 3.018, P = 0.0071. e Latency to enter bright side; unpaired t-test (one-tailed), t₁₁ = 1.858, P = 0.0450, EYFP n = 7, ARCH n = 6 mice. Data presented as mean ± SEM, ** P < 0.01, * P < 0.05