Fig. 5.

DP CD8 T cells are highly clonal and share little overlap with other CD8 TILs or peripheral CD8 T cells. a Diversity of the TCRβ repertoire within blood memory CD8 T cells, DN, SP, and DP CD8 TILs. The frequencies of the most frequent clonotype, the 2nd to 5th most frequent, the 6th to 30th most frequent, and the rest of the clonotypes (others) are shown for a HPV-positive HNSCC patient, a HPV-negative HNSCC patient, an ovarian cancer patient, and a melanoma patient. b The 500 most frequent CD8 TIL clonotypes for each subset are plotted based on their frequency in DN, SP, and DP CD8 TILs. Each dot represents a distinct TCR clonotype. Dots on the axis indicate the clonotypes detected within a single repertoire; purple dots indicate clonotypes shared between two CD8 T-cell populations. c Same analysis as in b comparing the frequency of the top 500 clones in memory CD8 T cells in peripheral blood and uninvolved LN to the frequency in DN and DP CD8 TIL subsets. d Similarity between the TCR repertoires of CD8 T-cell subsets was measured using the Morisita–Horn index on six cancer patients connected with a dashed line