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. 2018 Jul 13;9(7):779. doi: 10.1038/s41419-018-0807-3

Fig. 4. miR-133b targets TGFBRI and TGFBRII.

Fig. 4

a Western blotting of TGFBRI and TGFBRII expression in the indicated cells. α-Tubulin served as the loading control. b Real-time PCR analysis of TGFBRI and TGFBRII expression in the indicated PCa cells. Transcript levels were normalized by GAPDH expression. Error bars represent the mean ± s.d. of three independent experiments. *P < 0.05. c–e Luciferase assay of cells transfected with pmirGLO-3′-UTR reporter in the indicated PCa cells, respectively. *P < 0.05. f–h MiRNP IP assay showing the association between miR-133b and TGFBRI and TGFBRII transcripts in PCa cells. Pulldown of IgG antibody served as the negative control