Fig. 5.

Requirement for CENP-A N terminus in centromere cohesion. a Peptide arrays encompassing the CENP-A N terminus (amino acids 1–126) probed with His-ATPsyn-α, followed by western analysis with an anti-His antibody (n = 3). Schematic of CENP-A N terminus showing position of conserved B1, B2 and B3 domains and histone fold domain. Table displays the amino acid identity of interacting peptides 8, 9, 10 and 19. b Schematic showing the position of GFP between amino acid 118 and 119 of CENP-A in GFP-CENP-A full length and GFP-CENP-A-Δ118 transgenes. c Top: quantitation of the number of centromere foci per S5/6 nucleus from fly lines expressing GFP-CENP-A or GFP-CENP-A-Δ118 (both homozygous insertions) in addition to endogenous CENP-A. Data pooled from three individual RNAi experiments. Significance was determined using an unpaired Student's t-test, ****p < 0.0001, **p < 0.01, *p < 0.05. Error bars = SEM. Bottom: representative images of S5/6 nuclei immuno-stained for CENP-C (red) and CENP-A or GFP (green). White arrowhead indicates two sister centromeres. Number of centromere foci per nucleus is marked. Scale bar = 10 μm