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. Author manuscript; available in PMC: 2018 Jul 14.
Published in final edited form as: Anal Chem. 2017 Mar 24;89(7):4272–4279. doi: 10.1021/acs.analchem.7b00341

Figure 5.

Figure 5.

Multistep conjugation of cT20/AuNPs with Protein A and anticholera toxin. (A) Absorbance spectra of 13 and 30 nm cT20/AuNPs before and after Protein A and antibody conjugation. (B) Cartoon illustration of the final anti-CT/PA/cT20/AuNP bioconjugate. (C) Surface plasmon resonance sensorgram depicting formation of a ganglioside-impregnated supported lipid bilayer, followed by cholera toxin and AuNP recognition, with a 100% POPC bilayer in the reference channel. (D) SPR imaging results of cholera toxin and AuNP recognition across a lipid membrane array.