Fig. 1.

Loss of 5hmC is a hallmark of bladder cancer. a IHC staining of 5hmC in the positive control (normal kidney) and representative bladder cancer and normal bladder samples. Scale bar, 30 μm. b Analysis of 5hmC levels in bladder cancer and normal bladder samples represented by a 5hmC score. Statistical significance was determined by the Mann-Whitney U test. c Dot blot assay of 5hmC levels in normal bladder tissues relative to bladder cancer tissues. d Kaplan-Meier survival curves of bladder cancer patients with high and low 5hmC staining. P value was calculated by the log-rank test. e Multivariate Cox regression analyses of bladder cancer cases