Fig. 3.

Vitamin C treatment increases 5hmC levels and decreases malignant phenotypes in bladder cancer cells in vitro. a Dot blot assay of 5hmC levels in normal bladder and cancer cell lines. b The relative transcription levels measured by RT-qPCR of 5hmC-related genes and SVCTs in normal bladder and bladder cancer cells. c Dot blot assay of 5hmC levels of T24 cells at varying concentrations and time periods with vitamin C. d Dot blot assay of 5mC levels of T24 cells at varying concentrations and time periods with vitamin C. e MTS assay of cell viability for normal bladder and cancer cell lines at varying concentrations with vitamin C. f Apoptosis assay of T24 cells at varying concentrations with vitamin C. g Clone formation assay for normal bladder and cancer cell lines at varying concentrations with vitamin C. Statistical significance was determined by the Mann-Whitney U test