Table 2.
Performance of the Euroimmun ZIKV IgM-ELISA kit
| Performance according to the type of serum samples | ZIKV IgM-ELISA result | % (95% CI) | |
|---|---|---|---|
| Sensitivity | Positive | Negative | |
| Acute-phase samples of RT-PCR-confirmed Zika casesa | 0 | 14 | 0.0 (0.0–23.2) |
| Convalescent-phase samples of RT-PCR-confirmed Zika cases | 1 | 7 | 12.5 (0.3–52.7) |
| Specificity | Negative | Positive | |
| Acute-phase sample of RT-PCR-confirmed dengue cases | 58 | 0 | 100.0 (93.8–100.0) |
| Samples of DENV-1 infected cases | 19 | 0 | 100.0 (82.4–100.0) |
| Samples of DENV-2 infected cases | 19 | 0 | 100.0 (82.4–100.0) |
| Samples of DENV-4 infected cases | 20 | 0 | 100.0 (83.2–100.0) |
| Samples of primary dengue cases | 13 | 0 | 100.0 (75.3–100.0) |
| Samples of secondary dengue cases | 45 | 0 | 100.0 (92.1–100.0) |
| Convalescent-phase sample of RT-PCR-confirmed dengue cases | 58 | 1 | 98.3 (90.9–100.0) |
| Samples of DENV-1 infected cases | 20 | 0 | 100.0 (83.2–100.0) |
| Samples of DENV-2 infected cases | 18 | 1 | 94.7 (74.0–99.9) |
| Samples of DENV-4 infected cases | 20 | 0 | 100.0 (83.2–100.0) |
| Samples of primary dengue cases | 12 | 1 | 92.3 (64.0–99.8) |
| Samples of secondary dengue cases | 46 | 0 | 100.0 (92.3–100.0) |
| Blood donors samples | 23 | 0 | 100.0 (85.2–100.0) |
aAcute-phase serum samples were collected at a mean of 3 days post onset of symptoms. Thus, the ZIKV IgM-ELISA was not expected to be positive at that time