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Inflammatory response and renal injury. Renal cortical NLRP3 expression showed stepwise increase with the highest level in the HEAT+F group by quantitation of % NLRP3 positive stain area (a-c, 400×, Scale bar 50 μm). Caspase 3 expression was higher in heat stress + Fructose (HEAT+F) group compared to the other groups (d-f, 400× Scale bar 50 μm). Similar findings were shown for Interleukin 18 (g-i, 400×, Scale bar 50 μm). Key: *, p < 0.05; **, p < 0.01; ***p < 0.001, ****, p < 0.0001
