Table 2.
List of expression plasmid used in this study
| Plasmid | Description | Short name construct | Gene of interest | Reference |
|---|---|---|---|---|
| pPICZαA | -/PAOX/5′UTR/MFA/TAOX; ZeoR | – | b | |
| pPIC9Kα | -/PAOX/5′UTR/MFA/TAOX; his4, KanR | – | b | |
| pPNic384 | -/PAOX/5′UTR/MFA/TAOX; his4, KanR | IL8 | a | |
| pPNic775 | UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Kan R | IL8 | a | |
| pPNic948 | -/PAOX/5′UTR/MFA/TAOX; his4, ZeoR | Ref | IL8 | a |
| pPNic947 | UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Zeo R | UNall | IL8 | a |
| pPNIc706 | -/PAOX/5′UTR/MFA/TAOX; his4, KanR ZeoR | IL8 | a | |
| pPNic710 | -/PAOX/5′UTR/MFA/TAOX; his4, KanR, ZeoR | faeA | a | |
| pPNic957 | UNA1/PAOX/5′UTR/MFA/TAOX; his4, Kan R , Zeo R | UNA1 | faeA | a |
| pPNic958 | UNA2/PAOX/5′UTR/MFA/TAOX; his4, Kan R , Zeo R | UNA2 | faeA | a |
| pPNic959 | -/PAOX/UNB/MFA/TAOX; his4, KanR, ZeoR | UNB | faeA | a |
| pPNic964 | UNA1/UNA2/PAOX/5UTR/MFA/TAOX; his4, Kan R , Zeo R | UNA1 + UNA2 | faeA | a |
| pPNic961 | UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, KanR, ZeoR | UNall | faeA | a |
| pPNy051 | UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Kan R , Zeo R | – | – | a |
| pPNy063 | UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Kan R , Zeo R | UNall[EAEA] | faeA | a |
| pPNy060 | UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Kan R , Zeo R | – | – | a |
| pPNy059 | UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Kan R , Zeo R | UNall[EAYVEF] | faeA | a |
References: a: This study; b: Thermo Fisher
Structural elements: PAOX1: Promoter AOX1; 5′ UTR: 5′ Untranslated Region; UNA1, UNB and UNA2: regulatory element (see text); MFA: S. cerevisiae α-mating factor pre-pro leader sequence; TAOX; terminator AOX1; his4: P. pastoris histidine selection marker; KanR: G418 resistance selection marker; ZeoR: P. pastoris zeocin selection marker