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. 2018 Jul 10;13:4029–4043. doi: 10.2147/IJN.S163956

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© 2018 Wang et al. This work is published and licensed by Dove Medical Press Limited

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The influence of different topographies on mice BMSC osteogenic differentiation.

Notes: BMSCs were seeded on the blank plastic culture plates NC or titanium surface with different topographies in the complete culture medium. (A) After 3-day incubation, the mRNA expression of osteogenesis-related genes was analyzed. (B) After 1-week incubation, ALP staining and ALP activity were measured. (C) After 3-week incubation, alizarin red staining and semiquantitative analysis were performed. Means were compared with one-way analysis of variance combined with Bonferroni’s post-hoc test. *p<0.05; **p<0.01.

Abbreviations: ALP, alkaline phosphatase; BMP-2, bone morphogenetic protein-2; BMSC, bone marrow mesenchymal stem cell; Runx2, runt-related transcription factor 2; P, polished specimen; NC, negative control; NT-30, titanium nanotube anodized under 5 V; NT-100, titanium nanotube anodized under 20 V; OD, optical density.