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. 2018 Jul 10;13:4029–4043. doi: 10.2147/IJN.S163956

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© 2018 Wang et al. This work is published and licensed by Dove Medical Press Limited

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The effects of different topographies on macrophage polarization in vitro.

Notes: Mice BMDMs were seeded on NC or titanium surface with different topographies for 3-day culture. (A) The mRNA expression of M1 macrophage markers (iNOS, IL-6) and M2 macrophage markers (Arg-1, IL-10) of BMDMs in different groups. (B) Confocal images of M1 and M2 macrophage markers iNOS and CD163. Magnification ×200. (C) Quantitative analysis of confocal images. (D) The scatter plot of flow cytometry detection of M1 and M2 macrophage surface markers CCR7 and CD206. (E) The percentage of CCR7⁺ BMDMs (M1) and CD206⁺ BMDMs (M2) determined by flow cytometry. Means were compared with one-way analysis of variance combined with Bonferroni’s post-hoc test. *p<0.05; **p<0.01.

Abbreviations: BMDMs, bone marrow-derived macrophages; iNOS, inducible nitric oxide synthase; Arg-1, arginase-1; IL, interleukin; P, polished specimen; NC, negative control; NT-30, titanium nanotube anodized under 5 V; NT-100, titanium nanotube anodized under 20 V.