Skip to main content
. 2018 Jul 14;596(14):2883–2900. doi: 10.1113/JP275833

Figure 2. Effect of unloading and reloading on miRNAs (dystromirs) in adult and aged medial gastrocnemius (MG, top row) and tibialis anterior (TA, bottom row) muscles.

Figure 2

Expression of miR‐31 (A and D), miR‐146b (B and E) and miR‐374 (C and F) determined by RT‐qPCR and quantified using the ΔΔC T method with U87 used as a housekeeping gene. The inset in D reflects the percentage change in miR‐31 levels between day 1 of reloading (REL) and day 14 of unloading (UL). For adult and aged muscles n = 5–6/time point. In A and D there is a significant difference between age groups at all times P < 0.05. In B and E there is an interaction P ≤ 0.03 and main effect for age P ≤ 0.01. In F there is an interaction P ≤ 0.001 and main effect for age P ≤ 0.01. * P < 0.05 between age groups, † P < 0.05 vs. old baseline within group, # P < 0.05 vs. adult baseline within group. Data are presented as mean ± SEM.