Figure 6.

ROS production and structural changes (elastin arrangement and wall thickness) in the TA after L-NAME treatment in young SHR. DHE fluorescence detection (ex 540 nm/em 590 nm) was used to assess vascular redox homeostasis disruption in aortic cryosections, where ROS formation was determined by calculating the intensity (density/area) (a, b). Elastin autofluorescence (ex 488 nm/em 510 nm) was detected using fluorescence microscopy of aortic cryosections (c, d). Wall thickness: tunica intima + tunica media was determined in μm (e, f). E: endothelium; M: tunica media; A: adventitia; SHR: control group (n = 8); SHR + L-NAME: SHR treated with L-NAME (n = 7).