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. 2018 Jun 28;2018:3583921. doi: 10.1155/2018/3583921

Figure 1.

Figure 1

Effects of Tanshinone IIA (TSN) preconditioning on lactate dehydrogenase activity, cell viability, and 14-3-3η expression in H9c2 cells subjected to anoxia/reoxygenation (A/R). (a, b) Cells were treated in a dose-dependent manner with 2 μM to 32 μM for 24 hours prior to induction of A/R. (c, d, e, f) Cells were treated in a time-dependent manner from 12 hours to 48 hours prior to A/R. (e, f) The time-dependent effect of TSN preconditioning on 14-3-3η expression. Control group represents cells without any treatment. β-Actin served as a loading control. Values are presented as the mean ± SEM for five individual experiments. (A) P < 0.01 versus control group; (B) P < 0.01 versus A/R group; (C) P < 0.01 versus 1% DMSO group; (D) P < 0.05 versus A/R group; (E) P < 0.05 versus 1% DMSO group; (F) P < 0.01 versus 8 μM TSN + A/R (48 hours) group.