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. 2018 Jun 28;2018:3583921. doi: 10.1155/2018/3583921

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Copyright © 2018 Zeyu Zhang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Protein interaction among Bcl-2, 14-3-3η, and VDAC-1 on Tanshinone IIA (TSN) preconditioning in H9c2 cells subjected to A/R injury. (a) Cells were pretreated with TSN for 2 days prior to A/R injury and extraction of proteins. Proteins were immunoprecipitated with anti-14-3-3η, anti-Bcl-2, and anti-VDAC-1, respectively, and immunoblotted with anti-Bcl-2 and anti-VDAC-1. (b) Cells were pretreated with TSN for 2 days prior to A/R injury. The cyan color reflects overlap of VDAC-1 and 14-3-3η. A yellow color reflects overlap of VDAC-1 and Bcl-2, a purple color reflects overlap of 14-3-3η and Bcl-2, and a white color reflects overlap of 14-3-3η, Bcl-2, and VDAC-1. Data were collected from three individual experiments.