Figure 6.

Inhibiting mitochondrial translocation of Bcl-2 by silencing 14-3-3η expression abolished the protective effect of Tanshinone IIA (TSN) pretreatment on mPTP opening and cyt c release in H9c2 cells subjected to anoxia/reoxygenation (A/R) injury. Ca²⁺-induced swelling of mitochondria was used to access mPTP opening. mPTP opening led to mitochondrial swelling and caused a change in absorbance at 520 nm. (a) The changes in absorbance were detected every 2 min to access the progress of mPTP opening. (b) The extent of mPTP opening was accessed by using the equation ∆OD = A₅₂₀0 min − A₅₂₀20 min. (c, d) The cyt c release from mitochondria to the cytoplasm was accessed by Western blot analysis. Values are presented as the mean ± SEM for five individual experiments. (A) P < 0.01 versus control group; (B) P < 0.01 versus A/R group; (C) P < 0.05 versus A/R group; (D) P < 0.05 versus TSN + A/R group; (E) P < 0.01 versus TSN + A/R group; (E) P < 0.01 versus TSN + A/R group; (F) P < 0.01 versus AD14-3-3ηRNAi group.