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Representative cell cycle analysis in different treatment strategies. Combination of caffeine and TMZ impeded TMZ-induced G2 delay in U87-MG cells and caused an increase in the sub-G1 and MN phase (TMZ versus TC/CTC, p < 0.05). An additional increase in MN proportion but not sub-G1 was seen in pretreatment plus combination strategy. ∗, #, and & represent for p < 0.05 when compared to control, TMZ, and TC, respectively.
