Table 2. Effects of metal chelators on PtTPS activity.
| Chelators | Concentration (mM) | Specific activity (U/mg) | Residual activity (%) |
|---|---|---|---|
| None | 2.5 | 11.995 ± 0.07a | 100 |
| Sodium citrate | 2.5 | 11.273 ± 0.09b | 93.98 |
| Citric acid | 2.5 | 0.424 ± 0.20c | 3.55 |
| EDTA | 2.5 | 0.052 ± 0.03d | 0.43 |
| EGTA | 2.5 | 0.026 ± 0.04d | 0.22 |
| CDTA | 2.5 | 0.010 ± 0.02d | 0.08 |
Note:
TPS activity was assayed as introduced in the methods. All the experiments were done three times and the values indicate the mean ± SD. The different superscript letters indicate a significant difference. (P ≤ 0.05 according to Duncan’s multiple range test).