Table 1. Details of candidate reference genes, Accession number, primer sequences, amplicon size, PCR efficiency (%) and regression coefficient (R2) for each candidate reference gene selected in this study.
| Sr. no. | Gene label | Accession No. | Gene description | Primer sequence (5′-3′) | Amplicon size (bp) | PCR efficiency (%) | R2 |
|---|---|---|---|---|---|---|---|
| 1 | 18S | MH277331 | 18S rRNA | F-CCGTCCTAGTCTCAACCATAAAC R-GCTCTCAGTCTGTCAATCCTTG |
189 | 102.30% | 1 |
| 2 | ACT | MH279969 | Actin | F-ATCACACCTTCTACAACGAGC R-CAGAGTCCAACACGATACCAG |
207 | 92.03% | 0.994 |
| 3 | EF1α | MH310424 | Elongation Factor 1 α | F-AGCGTGTGATTGAGAGGTTC R-AGATACCAGCCTCAAAACCAC |
53 | 98.60% | 0.99 |
| 4 | UBQ | MH310425 | Ubiquitin | F-CACTTCGACCGCCACTAC R-AGGGCATCACAATCTTCACAG |
60 | 90.54% | 0.992 |
| 5 | RbcL | KP697362 | Ribulose 1,5-Bisphosphate Oxygenase/Carboxylase Large | F-ATGTCACCACAAACAGAGACTAAAGC R-GTAAAATCAAGTCCACCRCG |
530 | 97.69% | 0.996 |
| 6 | β-TUB | MH310423 | β-tubulin | F-ACCTCCATCCAGGAGATGTT R-GTGAACTCCATCTCGTCCATTC |
60 | 94.08% | 0.996 |
| 7 | GAPDH | MH279970 | Glyceraldehyde3-phosphate dehydrogenase | F-ACCACAGTCCATGCCATCAC R-TCCACCACCCTGTTGCTGTA |
264 | 96.78% | 0.99 |
| 8 | NHX | KU525079 | Sodium/proton antiporter | F-TGCTAGCTCTTGTCCTGATTG R-ATTGACACAGCACCTCTCATTA |
120 | 103.70% | 0.997 |