Table 2. geNorm analysis and ranking of candidate reference genes based on stability value (M).
Lower M value represents most stable reference genes and higher M value showed least stable reference genes.
| A. geNorm analysis for individual candidate reference genes | |||||
|---|---|---|---|---|---|
| Sr. No | Reference genes | Physiological tissue samples | Salt stress samples | ||
| Stability value (M < 1.5) | Ranking | Stability value (M < 1.5) | Ranking | ||
| 1 | 18S | 0.880 | 4 | 1.020 | 2 |
| 2 | ACT | 0.721 | 1 | 0.927 | 1 |
| 3 | EF1α | 0.761 | 2 | 1.021 | 3 |
| 4 | UBQ | 0.928 | 5 | 1.399 | 7 |
| 5 | RbcL | 1.225 | 6 | 1.357 | 6 |
| 6 | B-TUB | 0.763 | 3 | 1.351 | 5 |
| 7 | GAPDH | 1.599 | 7 | 1.257 | 4 |