Figure 1. Murine cohort design.

All mice in Cohorts 1–3 were on a C57Bl/6J background, maintained on corncob bedding under standard housing conditions and fed Teklad Global 18% Protein Rodent Diet unless indicated otherwise. (A) Cohort 1—Five breeding pairs were prepared from mice housed in one room (F₀ generation). Twenty-five breeding pairs were set up from the F₀ progeny (F₁ generation). Fifty-seven female and 57 male mice of F₂ generation were used for analysis of Cohort 1. Bedding was mixed among all F₂ cages in Cohort 1 beginning at weaning (three weeks old) until the diet switch to AIN-76A (6–12 weeks old). All mice were housed in one room. (B) Cohort 2—Twelve and seven age-matched male mice (19 total, 10–12 weeks old) were obtained from two separate C57Bl/6 breeding colonies, respectively which were housed in a single, yet separate room than Cohort 1. (C) Cohort 3—Thirty-six age-matched male mice (8–10 weeks old) were purchased from the Jackson Laboratories and acclimated for two weeks in a single, yet separate room than both Cohorts 1 and 2.