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Cytological assessment of vaginal smears stained by Crystal Violet (A) Proestrus: epithelial cells-mostly rounded but some cells showing early stages of cornification of approaching estrus (B) Estrus: large cornified cells in clumps (C) Metestrus: leucocytes with smaller numbers of non-nucleated epithelial cells (D) Diestrus: mainly leucocytes with small number of nucleated epithelial and cornified cells. Scale bars: 10 µm
