Table 3. Primer sequences.
Name | Forward primer(5'->3') | Reverse primer(5'->3') |
hsa-miR-506-3p | ACACTCATAAGGCACCCTTC | TCTACTCAGAAGGGGAGTAC |
hsa-miR-7162-3p | CACTCATCTGAGGTGGAAC | GCTGCTGTTCCGAGTACAT |
hsa-miR-125a-3p | ACTCACAGGTGAGGTTC | GGCTGGGAAGGAGAGTAC |
hsa-miR-200a-3p | CACTCTAACACTGTCTGG | ACATCGTTACCGAGAGTA |
hsa-miR-1291 | CACTCTGGCCCTGACTG | ACTGCTGGTCTTGAGTACA |
hsa-miR-3192-5p | ACACTCTCTGGGAGGTT | TTCCACTGCTAGAGTACAT |
hsa-miR-4441 | ACACTCTACAGGGAGGAG | TACAATCTCCGAGAGTAC |
hsa-miR-4728-5p | ACACTCTGGGAGGGGAGAG | TGCTTGCTGGAGAGTACA |
hsa-miR-4291 | ACTCTTCAGCAGGAACAG | AGCTGTTCCTGGAGAGTAC |
hsa-miR-548n | ACTCCAAAAGTAATTGTGG | ACAAAATCCACGAGAGTAC |
U6 | CTCGCTTCGGCAGCACA | ACGCTTCACGAATTTGC |
RAB3D | GACCTCCGGTTTAGAGGCAC | GTTGGTTGGTGTTTGGGAGC |
CDK4 | TGATGCGCCAGTTTCTAAGAGG | GGTCGGCTTCAGAGTTTC |
MMP9 | CCTGGAGACCTGAGAACCAATC | CCACCCGAGTGTAACCATAGC |
β-actin | CATCCTGGCCTCGCTGT | GCTGTCACCTTCACCGTTCC |
All the reactions were carried out as described previously [27].