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. 2018 Jun 10;10(6):1239–1256. doi: 10.18632/aging.101463

Figure 6.

Figure 6

Humanin and MOTS-c modulate the mitochondrial respiration. (A) Cellular oxygen consumption rate (OCR; pmole/min/total DNA) in non-senescence (quiescent) and senescent cells in the absence or presence of either HNG (a potent analogue of humanin with a glycine substitution, S14G) or MOTS-c. The basal respiration, spare respiratory capacity, and ATP production are calculated based on the sequential compound injection according to the manufacture’s instruction. (B) Representative western blots of carnitine palmitoyltransferase I (CPT1A) in non-senescence and senescent cells in the absence or presence of either HNG (a potent analogue of humanin with a glycine substitution, S14G) or MOTS-c. Quantification of carnitine palmitoyltransferase I (CPT1A) expression. Data are reported as mean ± SEM of three independent experiments. Significant differences were determined with one-way ANOVA followed by Tukey’s post hoc test. *p<0.05.