Figure 2. Energy balance in A20^flRR mice is associated with an elongated small intestine.

(A, B) Quantification of secretory cells by immunofluorescence analysis of RR and A20^flRR mice; secretory cell types in red identified by staining for Muc2 (goblet cells), DCLK1 (tuft cells), ChromograninA (ChgA, enteroendocrine cells) and lysozyme (Paneth cells), EpCAM in green, DAPI in blue. (C–F) Metabolic parameters of R+ and A20^flR+ mice. (C) Oxygen consumption rate measured using CLAMS cages. (D) Food intake during a 4 day period. (E) Lean and adipose weight determined by EchoMRI. (F) Fecal energy content measured using bomb calorimetry. (G and H) Small intestine (SI) length (G) and representative image (H) of 8–12 week old R+, A20^flR+, RR and A20^flRR mice. The black bar indicates difference in SI length. Scale bars; 100 μm. Data from one experiment representative of at least three independent experiments (A–E, G, H) or from one experiment (F). (E, mean and s.e.m., n = 10–11). **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant by Mann-Whitney U or one-way ANOVA.