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. 2018 Jul 9;8:232. doi: 10.3389/fcimb.2018.00232

Figure 4.

Figure 4

Insertions of large fragments had an adverse effect on PCV2 VLPs assembly. (A) Simulated 3D structures of foreign epitopes (Cap-M5 and M6) on the surface of the PCV2 capsid. Images on bottom of (A) show top views (high magnification) of foreign epitopes on a plateau formed by two neighboring MP-Lcd. Foreign epitopes were labeled in orange; MP-Lcd was labeled in red and Loop CD, except MP-Lcd was labeled in green and Loop BC-decorated five-fold axes of the icosahedra were labeled in blue on PCV2 capsid. (B) Morphologies of PCV2 Cap-M5 and -M6 after dialysis against assembly buffer. Morphologies were observed by TEM after samples were negatively stained.