Table 1. Primer sequences in Real-time PCR.
Target gene | Strand | Primer sequence |
β-actin | Forward | 5ʹ-TCCCTG GAGAAGAGCTACG-3ʹ |
Reverse | 5ʹ-GTAGTTTCGTGGATGCCACA-3ʹ | |
PTPN22 | Forward | 5ʹ-CCAGCTACATCAATGCCAACTTC-3ʹ |
Reverse | 5ʹ-CCAAATCATCCTCCAGAAGTCC-3ʹ | |
miR-181a | Target sequence | 5ʹ-AACAUUCAACGCUGUCGGUGAGU-3ʹ |
miR-181b | Target sequence | 5ʹ-AACAUUCAUUGCUGUCGGUGGGU-3ʹ |