Table 2. The effect of different variables on particle size and size SPAN (preliminary tests) .
| Independent variables | Dependent variables | |||||
| Stearic acid (mM) | Phospholipon ® 90G(mM) | Sodium taurocholate (mM) | Mixing | Addition method | Particle size (µm) | Size SPAN |
| 0.35 | 0.07 | 0.037 | 700rpm | Pour | 7.1 | 4.62 |
| 0.35 | 0.07 | 0.037 | 900rpm | Pour | 4.06 | 4.66 |
| 0.35 | 0.07 | 0.037 | 1200rpm | Pour | 1.83 | 2.2 |
| 0.35 | 0.07 | 0.037 | Bath sonic.* | Pour | 1.88 | 3.87 |
| 0.35 | 0.07 | 0.037 | Probe sonic. | Pour | 0.705 | 1.95 |
| 0.5 | 0.07 | 0.037 | 1200rpm | Pour | 0.806 | 3.01 |
| 0.5 | 0.07 | 0.037 | Bath sonic. | Pour | 0.91 | 2.48 |
| 0.5 | 0.07 | 0.037 | Probe sonic. | Pour | 0.64 | 0.89 |
| 0.75 | 0.07 | 0.037 | Probe sonic. | Pour | 12.83 | 6.37 |
| 1 | 0.07 | 0.037 | Probe sonic. | Pour | Aggregation occurred | |
| 0.5 | 0.07 | 0.025 | Probe sonic. | Pour | 9.53 | 3.19 |
| 0.5 | 0.07 | 0.03 | Probe sonic. | Pour | 0.38 | 0.77 |
| 0.5 | 0.07 | 0.045 | Probe sonic. | Pour | 1.96 | 2.05 |
| 0.5 | 0.07 | 0.037 | 1200rpm | Slowly added | 0.52 | 0.375 |
| 0.5 | 0.07 | 0.037 | Bath sonic. | Slowly added | 0.59 | 0.59 |
| 0.5 | 0.07 | 0.037 | Probe sonic. | Slowly added | 0.23 | 0.41 |
| 0.5 | 0.07 | 0.037 | 1200rpm | injected | 0.641 | 0.53 |
| 0.5 | 0.07 | 0.037 | Bath sonic. | injected | 1.47 | 0.717 |
| 0.5 | 0.07 | 0.037 | Probe sonic. | injected | 0.45 | 0.501 |
* Sonication