Table 3. The dependent and Independent variables, experimental design matrix and results of D-optimal design.

Run	Independent variables			Dependent variables
	A	B	C	Y1	Y2	Y3	Y4
	SA/Phos In range a	NA/T In range a	Mixing In range a	Particle size * Minimize a	Size SPAN	Zeta potential In range a	%EE Maximize a
1	6.80	12.50	bath	0.469	0.59	- 54.5	20.65
2	5.00	12.92	stirrer	0.63	3.61	- 71.2	19.54
3	5.00	12.92	stirrer	0.662	3.65	- 71.8	21.87
4	5.00	15.00	probe	0.389	1.59	- 51.5	26.43
5	6.45	15.00	stirrer	0.529	0.64	- 55.6	11.91
6	5.00	10.00	bath	0.07	0.73	- 49.1	21.59
7	7.19	10.00	stirrer	-	2.33	- 127.5	24.99
8	5.00	15.00	probe	0.435	0.55	- 51.1	28.66
9	6.03	10.00	stirrer	0.57	2.31	- 65.7	6.44
10	7.50	10.00	probe	1.976	3.76	- 83.6	22.68
11	6.25	10.00	bath	0.495	0.54	- 55.3	17.54
12	5.00	15.00	bath	0.577	1.35	- 65.5	16.29
13	6.25	12.50	probe	0.088	0.74	- 46.2	27.17
14	7.50	12.08	stirrer	0.608	4.19	- 68.1	17.05
15	5.31	10.63	probe	0.068	0.69	- 49.8	23.12
16	7.50	15.00	probe	0.112	0.69	- 41.5	37.24
17	6.25	12.39	stirrer	0.162	4.21	- 46.9	21.20
18	7.50	10.00	bath	6.701	0.64	- 91.4	21.22
19	6.45	15.00	stirrer	0.494	2.19	- 55.1	14.98
20	7.50	15.00	probe	0.095	0.75	- 40.3	33.41
21	7.50	15.00	bath	0.231	0.37	- 48.2	24.65
22	5.00	15.00	bath	0.547	0.83	- 62.4	13.40

SA/Phos (stearic acid to Phospholipon^®90G ratio); NA/T (nicotinamide to sodium taurocholate ratio); %EE=%Encapsulation efficiency.

* reported in µm

^a Constrains