Table 4. Analysis of variance for D-optimal refined models and the suggested independent variables for optimum preparation condition.
| D-optimal design | Source of variation | Sum of Squares | Degree of freedom | Mean Square | F value | P>F | |
| Particle size | Model | 4.55 | 9 | 0.41 | 32.14 | <0.0001 | Significant |
| Residual | 0.14 | 11 | 0.015 | ||||
| Pure Error | 4.676E-003 | 5 | 9.352E-004 | ||||
| Zeta potential | Model | 6760.32 | 11 | 614.57 | 4.42 | 0.0132 | Significant |
| Residual | 1391.86 | 10 | 139.19 | ||||
| Pure Error | 5.91 | 5 | 1.18 | ||||
| Entrapment efficiency | Model | 806.01 | 11 | 73.27 | 3.46 | 0.0303 | Significant |
| Residual | 212.00 | 10 | 21.20 | ||||
| Pure Error | 21.47 | 5 | 4.29 | ||||
| Suggested suituation | |||||||
| Observed | Predicted | % Observed to predicted ratio | |||||
| SA/Phos= 7.5 | size | 0.107 | 0.103 | 103.819 | |||
| NA/T= 14.74 | Zeta Potential | -40.90 | -40.30 | 101.489 | |||
| Mixing: Probe sonicaton | %EE | 36.026 | 35.277 | 102.123 | |||
SA/Phos (stearic acid to Phospholipon®90Gratio); NA/T (nicotinamide to sodium taurocholate ratio)