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. 2018 Jul 9;11:232. doi: 10.3389/fnmol.2018.00232

Figure 2.

Figure 2

hNav1.1-L1670W is a rescuable folding/trafficking defective mutant. Representative families of whole-cell sodium current traces recorded applying depolarizing steps from −70 to +60 mV in 5 mV increments (from a holding potential of −120 mV) in tsA-cells transfected with hNav1.1-WT and incubated at 37°C (A) transfected with hNav1.1-L1670W and incubated at 37°C (B) transfected with hNav1.1-WT and incubated at 30°C (C) transfected with hNav1.1-L1670W and incubated at 30°C (D); Scale bars: 1 nA, 1 ms. (E) Data and box chart (the bar represents the median, the square the mean, the box the SEM and the whiskers the 10–90% range) of the mean maximum current density of hNav1.1-WT incubated at 37°C (106.9 ± 21.0 pA/pF, n = 11), hNav1.1-L1670W incubated at 37°C (24.7 ± 1.7 pA/pF, n = 29), hNav1.1-WT incubated at 30°C (167 ± 27 pA/pF, n = 21), and hNav1.1-L1670W incubated at 30°C (89.7 ± 12.5 pA/pF, n = 22); statistical comparison: hNav1.1-WT-37°C vs. hNav1.1-L1670W-37°C p = 1.2 * 10−5, hNav1.1-WT-37°C vs. hNav1.1-WT-30°C n.s., hNav1.1- L1670W-37°C vs. hNav1.1-L1670W-30°C p = 8 * 10−7, hNav1.1-WT-30°C vs. hNav1.1-L1670W-30°C n.s. (Kruskall-Wallis test followed by Mann-Whitney test with Bonferroni correction for four comparisons). ns, non significant; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.(F) Fold increase in current density with incubation at 30°C.