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. 2018 Apr 23;193(2):194–206. doi: 10.1111/cei.13132

Figure 5.

Figure 5

Effect of PR‐957 on inflammatory cytokines in ischaemic brain following middle cerebral artery occlusion (MCAO). (a) Cytokines in the supernatants of brain tissues from the penumbra area (or complementary region in sham‐operated mice) were analysed using enzyme‐linked immunosorbent assay (ELISA). Interleukin (IL)‐1α, IL‐1β, IL‐2, IL‐4, IL‐6, IL‐10, IL‐12, IL‐17A, interferon (IFN)‐γ, tumour necrosis factor (TNF)‐α, granulocyte colony‐stimulating factor (GCSF) and granulocyte–macrophage colony‐stimulating factor (GM‐CSF) were included in the panel of analysed factors (n = 6 mice per group). (b–g) Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) was used to detect mRNA expression of IL‐1β (b), IL‐6 (c), TNF‐α (d), IL‐12 (e), IL‐23 (f) and IL‐17 (g), in the indicated groups. Same labelling conventions as previous figures (n = 6 mice per group). Bars and error bars represent means ± standard error of the mean (s.e.m.); # P < 0·05 compared with sham; *P < 0·05 versus the MCAO + vehicle group. The experiment was repeated twice with similar results.