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Comparison of the levels of herpes simplex type 1 (HSV‐1) replication in third‐trimester human placentas. Villous and decidual explants were infected with laboratory strain of HSV‐1 in vitro. HSV‐1 DNA was detected and quantified in the placental tissues using the HSV‐1 polymerase chain reaction (PCR) kit using quantitative real‐time (qRT)–PCR under the following conditions: denaturation at 95°C for 10 min, followed by 45 cycles of 95°C for 5 s and 60°C for 40 s.
