Table 9.
Methods for quantification of sugars in tomato by HPLC.
| Reference | Sample | Column; | Eluent | Detection | Repeatabilityb |
|
|---|---|---|---|---|---|---|
| preparation | column oven temperaturea | intra-day | inter-day | |||
| This study | NH2 SPE column | Nucleodur NH2 5 μm 250 × 4.6 mm; 30 °C | ACN/H2O = 80/20 |
RI | F: 2 G: 3 |
F: 4 G: 4 |
| Gancedo and Luh [12] | SEP PAC C18 SPE column | Micro-Bondapak carbohydrate 4 300 × 4 mm; RT | ACN/H2O = 80/20 |
RI | nr | nr |
| Yelle et al. [13] | C18 SPE column | P/10 carbohydrate 250 × 4.6 mm; nr | MeOH/H2O/ NH4OH = 79/20/1 |
RI | nr | nr |
| Vermeir et al. [17] | – | Aminex HPX-87C; 80 °C | H2O | RI | F: 9c G: 11c |
nr |
| Saito et al. Saito et al. [28] | – | TSK-GEL Amide-80 250 × 4.6 mm; 80 °C | ACN/H2O = 75/25 | RI | nr | nr |
| Zushi and Matsuzone [23] | – | Shim-pack SCR-101C 300 × 7.9 mm; 80 °C | H2O | RI | nr | nr |
a
RT, room temperature; nr: not reported.
b
The repeatability is reported as relative SD in %. F, repeatability for fructose; G: repeatability for glucose; nr, not reported.
c
The reported levels are unusually small: 2.00–2.43 g/l for glucose and 1.81–2.46 g/l for glucose.